Publications

Corresponding author(s) *

Hypoblast from human pluripotent stem cells regulates epiblast development. Okubo T, Rivron N, Kabata M, Masaki H, Kishimoto K, Semi K, Nakajima-Koyama M, Kunitomi H, Kaswandy B, Sato H, Nakauchi H, Woltjen K, Saitou M, Sasaki E, Yamamoto T, Takashima Y.Hypoblast from human pluripotent stem cells regulates epiblast development. Okubo T, Rivron N, Kabata M, Masaki H, Kishimoto K, Semi K, Nakajima-Koyama M, Kunitomi H, Kaswandy B, Sato H, Nakauchi H, Woltjen K, Saitou M, Sasaki E, Yamamoto T, Takashima Y*. Nature. Dec 5, 2023. doi: 10.1038/s41586-023-06871-2. PMID: 38052228.

Changing the public perception of human embryology. Rivron NC*, Martinez-Arias A, Sermon K, Mummery C, Schöler HR, Wells J, Nichols J, Hadjantonakis AK, Lancaster MA, Moris N, Fu J, Sturmey RG, Niakan K, Rossant J, Kato K. Nature Cell Biology. 2023 Dec;25(12):1717-1719. doi: 10.1038/s41556-023-01289-4. PMID: 37985870.

An update on human pre- and peri-implantation development: a blueprint for blastoids. David L*, Bruneau A, Fréour T, Rivron N, Van de Velde H. Current Opinion Genetic Development. 2023 Dec;83:102125. doi: 10.1016/j.gde.2023.102125. Epub 2023 Oct 4. PMID: 37801801.

An ethical framework for human embryology with embryo models. Rivron NC*, Martinez Arias A, Pera MF, Moris N, M'hamdi HI. Cell. 2023 Aug 17;186(17):3548-3557. doi: 10.1016/j.cell.2023.07.028. PMID: 37595564

Protocol for capturing trophectoderm stem cells reflecting the blastocyst stage. Seong J, Rivron NC*. STAR Protoc. 2023 Mar 16;4(2):102151. doi: 10.1016/j.xpro.2023.102151. Online ahead of print. PMID: 36930647

Generating human blastoids modeling blastocyst-stage embryos and implantation. Heidari Khoei H, Javali A, Kagawa H, Sommer TM, Sestini G, David L, Slovakova J, Novatchkova M, Scholte Op Reimer Y, Rivron N*. Nature Protocol. 2023 Feb 15. doi:10.1038/s41596-023-00802-1. PMID: 36792779 Review.

Going high and low: on pluralism and neutrality in human embryology policy-making. Ismaili M'hamdi H*, Rivron NC, Asscher EC. J Med Ethics. 2022 Dec 8:jme-2022-108515. doi: 10.1136/jme-2022-108515. PMID: 36600611

In preprints: improving and interrogating embryo models. Scialdone A, Rivron N. Development. 2022 Dec 1;149(23):dev201404. doi: 10.1242/dev.201404. PMID: 36475526

Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells. Pham TXA, Panda A, Kagawa H, To SK, Ertekin C, Georgolopoulos G, van Knippenberg SSFA, Allsop RN, Bruneau A, Chui JS, Vanheer L, Janiszewski A, Chappell J, Oberhuemer M, Tchinda RS, Talon I, Khodeer S, Rossant J, Lluis F, David L, Rivron N, Balaton BP, Pasque V*. Cell Stem Cell. 2022 Sep 1;29(9):1346-1365.e10. doi: 10.1016/j.stem.2022.08.001. PMID: 36055191

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation. Kagawa H, Javali A, Heidari Khoei H, Sommer TM, Sestini G, Novatchkova M, Scholte Op Reimer Y, Rivron N*. J Vis Exp. 2022 Aug 10;(186). doi: 10.3791/63388. PMID: 36036618

Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction. Zijlmans DW, Talon I, Verhelst S, Bendall A, Van Nerum K, Javali A, Malcolm AA, van Knippenberg SSFA, Biggins L, To SK, Janiszewski A, Admiraal D, Knops R, Corthout N, Balaton BP, Georgolopoulos G, Panda A, Bhanu NV, Collier AJ, Fabian C, Allsop RN, Chappell J, Pham TXA, Oberhuemer M, Ertekin C, Vanheer L, Athanasouli P, Lluis F, Deforce D, Jansen JH, Garcia BA, Vermeulen M, Rivron N, Dhaenens M, Marks H, Rugg-Gunn PJ, Pasque V*. Nature Cell Biology. 2022 Jun;24(6):858-871. doi: 10.1038/s41556-022-00932-w. PMID: 35697783

20 years of Developmental Cell: Looking forward. Hiiragi T, Gladfelter AS, Miguel-Aliaga I, Coller HA, Lau OS, McBride HM, Aoki K, Linkermann A, Santaguida S, Wasteneys G, Rivron NC, Shahbazi MN, Ebisuya M, Lancaster MA. Dev Cell. 2021 Dec 6;56(23):3185-3191. doi: 10.1016/j.devcel.2021.11.017. PMID: 34875224

Human blastoids model blastocyst development and implantation. Kagawa H, Javali A, Khoei HH, Sommer TM, Sestini G, Novatchkova M, Scholte Op Reimer Y, Castel G, Bruneau A, Maenhoudt N, Lammers J, Loubersac S, Freour T, Vankelecom H, David L, Rivron N*. Nature. doi: 10.1038/s41586-021-04267-8. Epub 2021 Dec 2. PMID: 34856602

A probabilistic framework for cellular lineage reconstruction using integrated single-cell 5-hydroxymethylcytosine and genomic DNA sequencing. Wangsanuwat C, Chialastri A, Aldeguer JF, Rivron NC, Dey SS*. Cell Rep Methods. 2021 Aug 23;1(4):100060. doi: 10.1016/j.crmeth.2021.100060. Epub 2021 Jul 30. PMID: 34590075

How will our understanding of human development evolve over the next 10 years? Brivanlou AH, Rivron N, Gleicher N. Nat Commun. 2021 Jul 29;12(1):4614. doi: 10.1038/s41467-021-24794-2. PMID: 34326327.

ISSCR Guidelines for Stem Cell Research and Clinical Translation: The 2021 update. Lovell-Badge R, Anthony E, Barker RA, Bubela T, Brivanlou AH, Carpenter M, Charo RA, Clark A, Clayton E, Cong Y, Daley GQ, Fu J, Fujita M, Greenfield A, Goldman SA, Hill L, Hyun I, Isasi R, Kahn J, Kato K, Kim JS, Kimmelman J, Knoblich JA, Mathews D, Montserrat N, Mosher J, Munsie M, Nakauchi H, Naldini L, Naughton G, Niakan K, Ogbogu U, Pedersen R, Rivron N, Rooke H, Rossant J, Round J, Saitou M, Sipp D, Steffann J, Sugarman J, Surani A, Takahashi J, Tang F, Turner L, Zettler PJ, Zhai X. Stem Cell Reports. 2021 Jun 8;16(6):1398-1408. doi: 10.1016/j.stemcr.2021.05.012. PMID: 34048692.

Human embryo research, stem cell-derived embryo models and in vitro gametogenesis: Considerations leading to the revised ISSCR guidelines. Clark AT, Brivanlou A, Fu J, Kato K, Mathews D, Niakan KK, Rivron N, Saitou M, Surani A, Tang F, Rossant J. Stem Cell Reports. 2021 Jun 8;16(6):1416-1424. doi: 10.1016/j.stemcr.2021.05.008. PMID: 34048690

SnapShot: Embryo models. Rivron N, Fu J. Stem Cell Reports. 2021 May 11;16(5):1142-1142.e1. doi: 10.1016/j.stemcr.2021.04.012. PMID: 33979599.

It takes a village to form embryo models. Rivron N, Fu J. Stem Cell Reports. 2021 May 11;16(5):1011-1013. doi: 10.1016/j.stemcr.2021.04.014. PMID: 33979590.

Induction of Human Trophoblast Stem Cells from Somatic Cells and Pluripotent Stem Cells. Castel G, Meistermann D, Bretin B, Firmin J, Blin J, Loubersac S, Bruneau A, Chevolleau S, Kilens S, Chariau C, Gaignerie A, Francheteau Q, Kagawa H, Charpentier E, Flippe L, François-Campion V, Haider S, Dietrich B, Knöfler M, Arima T, Bourdon J, Rivron N, Masson D, Fournier T, Okae H, Fréour T, David L*. Cell Rep. 2020 Nov 24;33(8):108419. doi: 10.1016/j.celrep.2020.108419. PMID: 33238118.

In Vitro Capture and Characterization of Embryonic Rosette-Stage Pluripotency Between Naive and Primed States. Alex Neagu, Emiel van Genderen, Irene Escudero, Lucas Verwegen, Dorota Kurek, Johannes Lehmann, Jente Stel, René A M Dirks, Guido van Mierlo, Alex Maas, Cindy Eleveld, Yang Ge, Alexander T den Dekker, Rutger W W Brouwer, Wilfred F J van IJcken, Miha Modic, Micha Drukker, Joop H Jansen, Nicolas C Rivron, Esther B Baart, Hendrik Marks, Derk Ten Berge*. Nat Cell Biol . 2020 May;22(5):534-545. doi: 10.1038/s41556-020-0508-x.

Accompanied by a News and views by Carolyn E. Dundes & Kyle M. Loh. Bridging naïve and primed pluripotency.

Toward Guidelines for Research on Human Embryo Models Formed From Stem Cells. Insoo Hyun, Megan Munsie, Martin F Pera, Nicolas C Rivron, Janet Rossant. Stem Cell Reports . 2020 Feb 11;14(2):169-174. doi: 10.1016/j.stemcr.2019.12.008.

Embryonic signals perpetuate polar-like trophoblast stem cells and pattern the blastocyst axis. Frias-Aldeguer J, Kip M, Vivié J, Li L, Alemany A, Korving J, Darmis F, van Oudenaarden A, Geijsen N, Rivron NC*. BioRxiv 510362; doi: https://doi.org/10.1101/510362.

Chemically-defined induction of a primitive endoderm and epiblast-like niche supports post-implantation progression from blastoids. Vrij EJ, Scholte op Reimer YS, Frias Aldeguer J, Misteli Guerreiro I, Kind J, Koo BK, van Blitterswijk CA, Rivron NC*. BioRxiv 510396; doi: https://doi.org/10.1101/510396.

Debate ethics of embryo models from stem cells. Rivron N*, Pera M*, Rossant J, Martinez Arias A, Zernicka-Goetz M, Fu J, van den Brink S, Bredenoord A, Dondorp W, de Wert G, Hyun I, Munsie M, Isasi R. Nature. 2018 Dec;564(7735):183-185. doi: 10.1038/d41586-018-07663-9.

Blastocyst-like structures generated solely from stem cells. Nicolas C Rivron*, Javier Frias-Aldeguer, Erik J Vrij, Jean-Charles Boisset, Jeroen Korving, Judith Vivié, Roman K Truckenmüller, Alexander van Oudenaarden, Clemens A van Blitterswijk #, Niels Geijsen # [# equal contribution]. Nature. 2018. 557, pages106–111 (2018). doi:10.1038/s41586-018-0051-0.

Formation of blastoids from mouse embryonic and trophoblast stem cells Nicolas C Rivron*. Protocol exchange. 2018. doi:10.1038/protex.2018.051.

Troy+ brain stem cells cycle through quiescence and regulate their number by sensing niche occupancy. Basak O, Krieger TG, Muraro MJ, Wiebrands K, Stange DE, Frias-Aldeguer J, Rivron NC, van de Wetering M, van Es JH, van Oudenaarden A, Simons BD, Clevers H*. Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):E610 E619. doi:10.1073/pnas.1715911114. PubMed PMID: 29311336.

Creating to understand - developmental biology meets engineering in Paris. Kicheva A, Rivron NC. Development. 2017 Mar 1;144(5):733-736. doi:10.1242/dev.144915. PubMed PMID: 28246208.

Directed assembly and development of material-free tissues with complex architectures. Vrij E, Rouwkema J, LaPointe V, van Blitterswijk C, Truckenmüller R, Rivron N*. Adv Mater. 2016 Jun;28(21):4032-9. doi: 10.1002/adma.201505723. PubMed PMID: 27000493.

3D high throughput screening and profiling of embryoid bodies in thermoformed microwell plates. Vrij EJ, Espinoza S, Heilig M, Kolew A, Schneider M, van Blitterswijk CA,Truckenmüller RK, Rivron NC*. Lab Chip. 2016 Feb21;16(4):734-42. doi: 10.1039/c5lc01499a. PubMed PMID: 26775648.

Engineered micro-objects as scaffolding elements in cellular building blocks for bottom-up tissue engineering approaches. Leferink A, Schipper D, Arts E, Vrij E, Rivron N, Karperien M, Mittmann K, vanBlitterswijk C, Moroni L, Truckenmüller R*. Adv Mater. 2014 Apr 23;26(16):2592-9. doi:10.1002/adma.201304539. PubMed PMID: 24395427.

Spheroid culture as a tool for creating 3D complex tissues. Fennema E, Rivron N, Rouwkema J, van Blitterswijk C, de Boer J. Trends Biotechnol. 2013 Feb;31(2):108-15. doi: 10.1016/j.tibtech.2012.12.003. Review. PubMed PMID: 23336996.

Tissue deformation spatially modulates VEGF signaling and angiogenesis. Rivron NC*, Vrij EJ, Rouwkema J, Le Gac S, van den Berg A, Truckenmüller RK,van Blitterswijk CA. Proc Natl Acad Sci U S A. 2012 May 1;109(18):6886-91. doi:10.1073/pnas.1201626109. Epub 2012 Apr 17. PubMed PMID: 22511716.

Sonic Hedgehog-activated engineered blood vessels enhance bone tissue formation. Rivron NC*, Raiss CC, Liu J, Nandakumar A, Sticht C, Gretz N, Truckenmüller R, Rouwkema J, van Blitterswijk CA. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4413-8. doi: 10.1073/pnas.1117627109. PubMed PMID: 22388744.

Fabrication of cell container arrays with overlaid surface topographies. Truckenmüller R, Giselbrecht S, Escalante-Marun M, Groenendijk M, Papenburg B,Rivron N, Unadkat H, Saile V, Subramaniam V, van den Berg A, van Blitterswijk C, Wessling M, de Boer J, Stamatialis D. Biomed Microdevices. 2012 Feb;14(1):95-107. doi:10.1007/s10544-011-9588-5. PubMed PMID: 22048776.

Thermoforming of film-based biomedical microdevices. Truckenmüller R, Giselbrecht S, Rivron N, Gottwald E, Saile V, van den Berg A, Wessling M, van Blitterswijk C. Adv Mater. 2011 Mar 18;23(11):1311-29. doi: 10.1002/adma.201003538. Review. PubMed PMID: 21400590.

Tissue assembly and organization: developmental mechanisms in microfabricated tissues. Rivron NC*, Rouwkema J, Truckenmüller R, Karperien M, De Boer J, VanBlitterswijk CA. Biomaterials. 2009 Oct;30(28):4851-8. doi:10.1016/j.biomaterials.2009.06.037. PubMed PMID: 19592088.

Vascularization in tissue engineering. Rouwkema J, Rivron NC, van Blitterswijk CA. Trends Biotechnol. 2008 Aug;26(8):434-41. doi:10.1016/j.tibtech.2008.04.009. Review. PubMed PMID: 18585808.